In vitro culture system of buffalo fetal fibroblasts ( BFF ) was investigated in this study.
本實驗探討了適合水牛胎兒成纖維細(xì)胞 ( BFF ) 體外培養(yǎng)的體系.
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Comparison of 10 % bFF and 10 % FBS with oocytes maturation; Efficiency of micro - drop culture was assessed.
對比 10%bFF 和10%胎牛血清(FBS),以及觀察微滴培養(yǎng)對卵母細(xì)胞成熟的影響.
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Therefore adding 10 % of mixed BFF in maturation medium is the best choice.
因此,成熟培養(yǎng)液中添加10%的混合BFF較為適宜.
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Therefore, adding 10 % of mixing BFF in maturation medium could be the best choice.
因此, 成熟培養(yǎng)液中以添加10%的混合BFF較為適宜.
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Among the three groups adding BFF at 10 % concentrationexpressed the best proliferous effect.
細(xì)胞的表型,以添加10%BFF的培養(yǎng)液促增殖效果最好.
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The fertilization inhibitory factors of BFF may be derived from serum rather than from follicular cells.
BFF的這種受精抑制因子可能來自血清而不是卵泡細(xì)胞.
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Shoulder pads may come and go, but a BFF is forever.
墊肩的流行趨勢來了又走, 但好朋友是永遠(yuǎn)的.
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In addition , the addition of 20 % bFF in maturation medium inhibited the hatching of blastocyst.
添加20%卵泡液的培養(yǎng)液對囊胚的孵化有抑制作用.
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Along with the increase of bFF, in IVF group , cleavage and blastocyst rate decreases.
添加D0血清體外受精胚胎卵裂率較高, 但對囊胚發(fā)育率影響差異不顯著(P>0.05).
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