Methods Porcinecornealendotheliawereobtainedwiththeepitheliaservingas control , CEN - Agandcornealepithelialantigen ( CEP - Ag ) wereisolatedandpurifiedby ion - exchangechromatographyandaffinity chromatographywith 2 B 4.14.1 monoclonalantibody. TherabbitswereimmunizedwiththeresultantCEN - AgandCEP - Ag andtheirseracollectedto harvestaCEN - Abandanti - cornealepithelialantibody ( aCEP - Ab ) by affinitychromatography.
方法用豬角膜內(nèi)皮做抗原,上皮做對(duì)照,經(jīng)離子交換層析及2B4.14.1單克隆抗體親和層析制得純化的CEN-Ag 和角膜上皮抗原 ( cornealepithelialantigen, CEP-Ag ),用提純的CEN-Ag和 CEP-Ag分別免疫家兔,取血清經(jīng)親和層析得到aCEN-Ab和抗角膜上皮抗原抗體(anti-cornealepithelialantibody, aCEP -Ab ).
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